Gum Arabic more than emulsiﬁer and food additive: a new experimental validation of anti-proliferative against colorectal colon cancer cell lines: Part 2, Dr. Ahmed A. M. Elnour, Mohamed E. S. Mirghani, Prof. N. A. Kabbashi, Md Z Alam, Djabir Daddiouaissa, Khalid H. Musa and Nour Hamid Abdurahman
Ahmed A. M. Elnour (abc*)
Mohamed E. S. Mirghani (ab)
N. A. Kabbashi (a)
Md Z Alam (a)
Djabir Daddiouaissa (a)
Khalid Hamid Musa (d)
Nour Hamid Abdurahman (e)
(a) Bioprocess & Molecular Engineering Research Unit (BPMERU), Biotechnology Engineering, Faculty of Engineering, International Islamic University, Malaysia (IIUM), Kuala Lumpur, Malaysia
(b) International Institute for Halal Research and Training (INHART), Kuala Lumpur, Malaysia
(c) Institute of Gum Arabic & Desertification Studies (IGADS), University of Kordofan, Sudan
(d) Food Science and Human Nutrition Department, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Kingdom of Saudi Arabia
(e) Centre of Excellence for Advanced Research in Fluid Flow (CARIFF), Universiti Malaysia Pahang Gambang, Malaysia
The aim of this paper is to discover the cytotoxicity activity of Acacia seyal gum and Prebio-Tcommercial (PTC) samples on human colon cancer (HCT-116) cell lines; in addition, its polyphenolic properties are investigated.
Approach: The methodology used in this paper involves fractionating methanol crude extracts of both Acacia seyal gum and Prebio-T-commercial, respectively. The cytotoxicity activity on human colon cancer cell lines for each fraction was studied using sulforhodamine B assay (SRB assay). Both samples, Acacia seyal gum and Prebio-T-commercial (PTC), were analysed precisely using high performance liquid chromatography (HPLC) technique.
Findings: Based on the analysis, more cytotoxicity activity was identified in the sample obtained from Acacia seyal gum (IC50=13.36µg/mL) compared to PTC (IC50=17.18µg/mL). Regarding the flavonoids content (FC), quercetin was the only flavonoids detected in the samples, found to be approximately of 4,640µg/100g for Acacia seyal gum, almost double the value of 2,560µg/100g for PTC.
Implications: The implications of this study are the evidence of flavonoids content and anti-cancer activity for both Acacia seyal gum and PTC used in folk medicine, as well as anticipation of cancer cell growth. It is expected that the proposed methods will give a noble contribution to the possible use of their bioactive compounds as natural anti-cancer agents.
Keywords: Cytotoxicity activity; Acacia seyal gum; Colorectal cancer (HCT-116); Flavonoids, and HPLC.