Preventive effect of black rice Ethanolic extract as natural antioxidants on some heavy metals induced disorders in experimental animal, Soheir N. Abd El-Rahman, Saad A. Mahagoub, Walid M. Shehata, Afaf A. Abd E. Shaaban
Soheir Nazmy Abd El-Rahman, Saad Aziz Mahagoub and Walid Mohamed Shehata, Agricultural Research Center, Egypt
Afaf Abd El-Raouf Abd El-Moneim Shaaban, Al-Azhar University, Egypt
Purpose: The present study investigated the protective effect of black rice ethanolic (60 and 80%) extract (BREE) on Drinking Water Heavy Metals (DWHM) induced liver injury, renal damage and alteration of lipid profile in male rats.
Design and methodology: Total phenols and total flavonoids in BREE were determined by HPLC. The study was conducted on 36 male Wistar rats weighing 193–195 g, the animals were divided into six equal groups. The first group was given Distilled Water (DW) and used as a control group (NC). The second group was administered DWHM (Mn 5 1000 ppm; Hg 5 10 ppm; Pb 5 100 ppm, Ni 5100 ppm, Fe 5 1200 ppm) and used as Positive Control (PC). The other groups of rats were administered BREE 60 and 80% (1 mg/1 ml) in DW and BREE 60% and 80% (1 mg/1 ml) in DWHM. Blood and tissue samples were collected after eight weeks. Lipid profile, hepatic markers, renal markers, Fasting Blood Sugar (FBS) and CA19.9 as the tumour marker for (GIT) were determined. Also, histological changes in kidney tissue were studied.
Findings: The results revealed that the rats treated with DWHM showed a significant (p # 0.05) increase in levels of LDL-C, TG, FBS, ALT, AST, urea, creatinine, uric acid and CA19.9 and significant (p # 0.05) decrease in HDL-C level. Heavy Metal (HM) intoxication induces some pathological alterations in the kidney as degeneration and large lumen. The rise in serum hepatic enzymes, LDL-C, TG, FBS, urea, creatinine and uric acid and histopathological changes were significantly attenuated by BREE 60 and 80%. Moreover, the level of serum HDL-C in BREE 60 and 80% (1 mg/ 1 ml DWHM) groups showed a significant (p # 0.05) increase as compared with PC.
Research implications: The current results ascertained the beneficial effects of BREE in controlling HMs induced disorders and the protection of liver and kidney against HMs intoxication in male rats.
Keywords: Black rice; Ethanolic extract; Heavy metals; HMs; Drinking water
Reference to this paper should be made as follows: Soheir, N.A., Saad, A.M., Walid, M.S. and Afaf, A.A.S. (2016) ‘Preventive Effect of Black Rice Ethanolic Extract as Natural Antioxidants on Some Heavy Metals Induced Disorders in Experimental Animal’, Int. J. Food, Nutrition and Public Health, Vol. 8, No. 1, pp.20–34.
