“Induction of Callus from nodal explant of Acacia Senegal”, Jummai T. Kaldapa and Njidda M Gadzama
Jummai T. Kaldapa and Prof. Njidda M. Gadzama
University of Maiduguri, Nigeria
Purpose: Acacia tree species are undoubtedly important for the rural poor in the Sahel†, but propagation through seed is often limited by poor seed selection and storage, exacerbated by the high mortality of seedlings in the nursery. In vitro micro propagation techniques provide an alternative way of developing high yielding tree species.
Design Methodology/Appproach: A protocol for callus induction was developed on nodal explants of Acacia Senegal, cultured on full and half strengths of MS media supplemented with different concentrations of 2,4-D (1.0–2.5mg/l), alone and in combination with 0.5mg/l kinetin.
Findings/Results: Results indicate prolific callus formation on full strength MS medium containing 2,4-D at all the concentrations. The addition of kinetin to the auxin enhanced calli formation, especially at concentrations 2.0mg/l 2,4-D +0.5mg/l kinetin. Calli morphology was friable and yellow white in colour. Half strength MS medium seems to have a delaying effect on induction period, with moderate formation of calli that are hard and brownish white. Callus induction is a promising pathway for tree improvement programmes.
Originality and Value: This work would be the first attempt to induce callus from nodal explants of Acacia Senegal in Borno State of Nigeria, with the view of obtaining alternative methods for generating reproducible protocol for future mass propagation of the tree crop in the State.
Keywords: Acacia Senegal; callus induction; induction capacity; Murashige and Skoog (MS); 2,4-Dichlorophenoxyaceticacid (2,4-D); Kinetin (KN)